FIG. 1.

PCR and RT-PCR of 10⁶ keratinocytes infected with KSHV/HHV8. (A) PCR with KS330 primers to test for the presence of viral DNA 6 days postinfection. Lanes: 1 and 2, two different cultures infected in parallel; 3, uninfected keratinocytes; 4, positive control (BC-3 cells). (B) PCR with KS330 primers to test the presence of KSHV/HHV8 genomes in infected keratinocytes 25 days postinfection. Lanes: 1 and 2, two different cultures infected in parallel; 3, uninfected keratinocytes; 4, BC-3 cells. (C) RT-PCR 6 days postinfection with primers specific for v-cyclin. Lanes: 1 and 3, RNA from two cell cultures infected in parallel; 2 and 4, RNA controls without reverse transcription showing no viral DNA contamination; 5, positive control (BC-3 cells); 6, uninfected keratinocytes; 7, primers with no template. (D) RT-PCR 6 days postinfection with primers specific for ORF K12. Lanes: 1 and 2, RNA from two cell cultures infected in parallel; 3, primers with no template; 4, positive control (BC-3 cells).