FIG. 2.

Nested PCRs with MCP outer and inner sets of primers. (A) Quantification by nested PCR of viral DNA extracted from purified particles. Lanes: 1 to 6, 10-fold serial dilutions from 2 × 10⁻³ to 2 × 10⁻⁸ amol/μl; 7, negative control (primers with no template). (B) Nested PCR of 10⁶ keratinocytes 8 weeks after infection with KSHV. Lanes: 1 to 3, three keratinocytes cultures independently infected; 4 and 5, uninfected keratinocytes; 6, negative control (primers with no template); 7, positive control (BC-3 cells).