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IFA of KSHV/HHV8-infected keratinocytes. (A and B) Detection of latent viral antigen. (A) Cells stained with anti-LANA MAb. Positive cells show speckled nuclear pattern. (B) Cells counterstained with DAPI to localize the nuclei. (C and D) Detection of lytic viral antigen after TPA induction. (C) Cells stained with MAb 11D1, raised against the ORF 59 product, DNA replication protein. Positive cells show characteristic nuclear staining. (D) Cells counterstained with DAPI.
