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Spindle shape acquisition of KSHV/HHV8 infected-keratinocytes as shown by phase contrast microscopy. (A) Primary human keratinocytes. (B) Infected keratinocytes after 4 weeks in culture (focus formation). (C) Infected keratinocytes after 4 weeks in culture (loss of contact inhibition). (D) Infected keratinocyte-forming colonies in soft agar. (E) Number of colonies grown in agar after plating 104 cells per plate (experiments were done in triplicate).
