Figure 5.

Effect of cDCV therapy with or without anti-CTLA-4 on T-cell infiltration in tumors. Tumors from the mice in Figure 4 were analyzed for the infiltration of T-cell subsets ((A) CD8+ T-cells and (B) CD4+ T-cells). Data were analyzed using Mann–Whitney U-test. *** p < 0.001, **** p < 0.0001. (C–F) Representative images of lung tumor nodules were analyzed for CD8⁺ cells using immunofluorescence staining. Arrows indicate the positive CD8⁺ stained cells in each field. cDCV: type 1 CD103⁺ dendritic cell vaccine; CTLA-4: cytotoxic T-lymphocyte antigen-4; CTRL: control; HPF: high-power field. All values reported represent means ± SEMs.