Figure 9: Cartoon depicting early and late defects associated with GARP dysfunction.

(Left) Control cells with normal Golgi morphology, Golgi enzymes, endosome-Golgi trafficking proteins, and component of trafficking machineries such as coats and SNAREs. (Middle) 3 h post-depletion of VPS54, the trans-side of Golgi enlarged, vesicle number is increased, endosome-Golgi trafficking proteins are mislocalized and redistributed to vesicles, Golgi-associated coat proteins, and SNAREs are decreased. (Right) Severe alteration of Golgi structure, depletion of Golgi enzymes, coat proteins, and SNAREs are a late response.