Table 2.
List of primary and secondary antibodies
| Antibody | Source/Catalog # | Species | WB dilution | IF dilution |
|---|---|---|---|---|
| ATP7A | Santa Cruz #Sc-376467 | Mouse | 1:500 | 1:300 |
| β-actin | Sigma, #A5441 | Mouse | 1:1000 | - |
| B4GALT1 | R&D Systems, AF-3609 | Goat | 1:500 | 1:300 |
| BET1L/GS15 | BD Biosciences #610961 | Mouse | - | 1:500 |
| BET1L/GS15 | This lab | Rabbit | - | 1:500 |
| BIG1 | Santa Cruz #sc-50391 | Rabbit | - | 1:300 |
| C1GALT1 | Santa Cruz # SC100745 | Mouse | 1:500 | 1:300 |
| Carboxypeptidase D/CPD | Kerafast # EB5001 | Rabbit | 1:1000 | 1:500 |
| Cathepsin D/CATD | Sigma, #C0715 | Mouse | 1:500 | - |
| CD-M6PR/MPR | Santa Cruz # sc365196 | Mouse | 1:1000 | - |
| CD-M6PR/MPR | Abcam # AB134153 | Rabbit | - | 1:1000 |
| CI-M6PR/IGF2R | Abcam # AB124767 | Rabbit | 1:2000 | 1:1000 |
| COPB2 | ABclonal, # A7036 | Rabbit | - | 1:400 |
| GALNT2 | Thermo Fisher # PA521541 | Rabbit | 1:1000 | - |
| GBF1 | BD transduction laboratories #612116 | Mouse | - | 1:500 |
| GM130/GOLGA2 | BD Biosciences, # 610823 | Mouse | - | 1:500 |
| GM130/GOLGA2 | CalBiochem, # CB1008 | Rabbit | - | 1:300 |
| GOSR1/GS28 | BD Biosciences # 611184 | Mouse | 1:500 | 1:500 |
| MGAT1 | Abcam, # ab180578 | Rabbit | 1:500 | - |
| MYC | Bethyl #A190-105A | Rabbit | 1:2000 | 1:1000 |
| MYC-Tag | Cell signaling #2276 | Mouse | 1:1000 | 1:500 |
| STX5 | Santa Cruz # sc-365124 | Mouse | - | 1:100 |
| STX6 | R&D Systems, #AF5664-SP | Sheep | 1:1000 | 1:400 |
| STX10 | ProteinTech #11036-I-AP | Rabbit | 1:1000 | - |
| STX16 | Abcam #AB134945 | Rabbit | 1:2000 | 1:1000 |
| TGN46/TGOLN2 | Bio-Rad, #AHP500G | Sheep | 1:2000 | - |
| TMEM87A | Novus #NBP1-90532 | Rabbit | 1:1000 | 1:400 |
| VAMP4 | Synaptic Sys # 136-002 | Rabbit | 1:500 | - |
| VPS51 | Sigma # HPA061447 | Rabbit | 1:1000 | - |
| VPS52 | Juan Bonifacino | Rabbit | 1:1000 | - |
| VPS53 | Thermo Fisher, #PA520548 | Rabbit | 1:1000 | - |
| VPS53 | Santa Cruz # sc514920 | Mouse | 1:500 | - |
| VPS54 | St John’s Lab, # STJ115181 | Rabbit | 1:1000 | - |
| VTI1A | BD # 611220 | Mouse | 1:500 | 1:300 |
| IRDye 680 anti-Mouse | LiCOR/926-68170 | Goat | 1:40000 | - |
| IRDye 800 anti-Rabbit | LiCOR/926-32211 | Goat | 1:40000 | - |
| IRDye 800 anti-Goat | LiCOR/926-32214 | Donkey | 1:40000 | - |
| Alexa Fluor 647 anti-Rabbit | Jackson Immuno Research/711-605-152 | Donkey | 1:500 | 1:1000 |
| Alexa Fluor 647 anti-Mouse | Jackson Immuno Research/715-605-151 | Donkey | 1:500 | 1:1000 |
| Alexa Fluor 647 anti-Goat | Jackson Immuno Research/ 705-605-147 | Donkey | - | 1:1000 |
| DyLight 647 anti-Sheep | Jackson Immuno Research/713-605-147 | Donkey | - | 1:1000 |
| Cy3-anti-Rabbit | Jackson Immuno Research/711-165-152 | Donkey | - | 1:1000 |
| Cy3-anti-Mouse | Jackson Immuno Research/715-165-151 | Donkey | 1:500 | 1:1000 |
| Alexa Fluor 488 anti-Rabbit | Jackson Immuno Research/711-545-152 | Donkey | 1:500 | 1:1000 |
| Alexa Fluor 488 anti-Mouse | Jackson Immuno Research/715-545-151 | Donkey | 1:500 | 1:1000 |