Table 2.
Optical trap summary.
| Parameter | 0 μM danicamtiv |
10 μM danicamtiv |
P-value |
|---|---|---|---|
| 10 uM ATP | |||
| Total Step (nm) | 4.9 ± 9.7 | 3.0 ± 9.0 | < 0.001 |
| Total Step (nm)* | 5.0 | 2.5 | |
| kf (s−1)* | 51 | 26 | |
| Detachment Rate (s−1) | 23 (−2.5/+2.5) | 24 (−0.8/+0.9) | 0.39 |
| Number of Events | 2076 | 4776 | |
| 1 mM ATP | |||
| Displacement (nm) | 5.1 ± 6.8 | 2.6 ± 7.2 | < 0.001 |
| Detachment Rate (s−1) | 41.4 (−6.6/+6.8) | 34.2 (−5.7/+7.1) | 0.15 |
| Number of Events | 965 | 1695 | |
| Isometric Force Clamp | |||
| k0 (s−1) | 61 (−21/+40) | 89 (−18/+24) | 0.12 |
| d (nm) | 1.40 (−0.37/+0.46) | 1.25 (−0.16/+0.17) | 0.35 |
| Number of Events | 1755 | 2107 |
*
ensemble average fit parameter