FIG. 5.
Immunohistochemistry of liver sections of C57BL/6 mice infected with the recombinant viruses SA59R13 (a), Penn98-1 (b), and S4R21 (c) and a mock-infected mouse (d) at day 5 p.i. Immunolabeling was performed as described for Fig. 4. Viral antigen staining was found in sinusoidal lining cells (consistent with endothelial and/or Kupfer cells) of livers infected with SA59R13 (a), Penn98-1 (b), and S4R21 (c). Viral antigen colocalized with focal necrosis areas in SA59R13 (a), confluent necrosis areas in Penn98-1 (b), and small clusters of hepatocytes in S4R21 (c). No signs of pathology or viral antigen were found in a mock-infected control (d). Magnification, ×200.