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[Preprint]. 2024 Oct 9:2024.10.08.617280. [Version 1] doi: 10.1101/2024.10.08.617280

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Figure 4. — (A) Phylogenetic analysis of C. steroidoreducens HCS.1 SDR domain-containing protein sequences. Product formed from 5β-dihydrocortisol by E. coli strains overexpressing SDR domain-containing proteins are shown with their respective gene identifiers. THC stand for tetrahydrocortisol. (B) Kinetic parameters of reduction of indicated steroid hormones by purified SDR domain-containing proteins. Abbreviations stand for dihydrotestosterone (DHT), dihydroprogesterone (DHP), dihydrocortisol (DHC), and dehydrolithocholic acid (DLCA).