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[Preprint]. 2024 Oct 13:2024.10.11.617929. [Version 1] doi: 10.1101/2024.10.11.617929

Figure 2|. Characterization of iEndothelial cells in 2D culture.

Figure 2|

(a) Immunostaining of ZO-1 (green) and F-actin (red) to quantify tight junctions’ property (b) Quantification of ZO-1 area fraction rate. iEC-Mut displayed lower area fractions than iEC-WT. (c) Gene expression showed (d) iEC-Mut exhibited lower gene expressions related to tight junctions (CLDN5, OCLN, and ZO-1) measured by PCR. n = 3. (e) Measurement of TEER indicating endothelial barrier functions. (f, g) Differential gene expression by RNAseq. n = 2. (h) GO enrichment analysis. Student’s t-test, **, p<0.01, * p<0.05.