Fig. 14. The 3-mer binds to UL141 interacting proteins.

3T3 or 293T cells were transfected with the four known human TRAIL receptors or CD155, using expression constructs where the receptor ectodomains were fused to a gpi-addition signal to facilitate cell-surface expression and avoid apoptosis mediated by overexpression of full-length TRAIL death receptors. Recombinant 3-mer protein engineered to express the indicated epitope tags on the individual subunits was then incubated with transfected cells (5μg/ml), and binding was detected using the indicated antibodies followed by flowcytometry.