Fig. 4. UL141 enhances the infectivity of the pentamer-null AD169 strain.

a, Schematic of the pentamer-null AD169 viruses used in the following experiments. b, ARPE-19 were infected with AD169 or UL141-restored AD169 (AD169¹⁴¹) at MOI 0.1. Cells were stained for IE1 at 5 and 10 dpi to measure the size of foci, or plaques in IE1+ nuclei per plaque. Each point in the bar graph represents a biological replicate (N=7–8). Data was logarithmically transformed to fit a Gaussian distribution prior to measuring statistical significance via Welch’s t test. c, Representative image of AD169 versus AD169¹⁴¹⁺ plaques in ARPE-19 cells at 10 dpi. Cells were stained for IE1 (green).