Fig. 5. UL116 is required for UL141-dependent entry and virion incorporation of the 3-mer.

a, Representative images of fibroblasts, epithelial cells, and endothelial cells following infection with UL116-sufficient versus UL116-deficient (Δ116) TB40 that encode UL141 or not (141− and Δ116¹⁴¹⁻; 141+ or Δ116¹⁴¹⁺). Fibroblasts and epithelial cells (ARPE-19) were infected with 50 genome equivalents/cell, while endothelial cells (HUVEC) were infected with 100 genome equivalents/cell. The cells were stained for IE1 and counterstained with Hoechst at 1 dpi to measure the percentage of infected cells in each condition. Each point represents a biological replicate. b, Western blot analysis of whole cell and crude virion lysates from 141+ or Δ116¹⁴¹⁺ infected fibroblasts. c, Immunofluorescence of the cytoplasmic viral assembly compartment (cVAC, white arrowheads) in fibroblasts infected with 141+ or Δ116¹⁴¹⁺ at 3 dpi (MOI 1). Scale bars represent 20 μm. d, Intensity profiles of UL141 (red) and gH (green) throughout the cVAC. The regions of interest used to measure the intensity profiles for each condition are represented by the yellow dashed arrows in c.