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[Preprint]. 2024 Oct 13:2024.10.13.617647. [Version 1] doi: 10.1101/2024.10.13.617647

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Fig. 6. — a, Schematic representation of the expression and purification process for the HCMV gH/UL116/UL141 complex. b, Size exclusion chromatography profile of the gH/UL116/UL141 complex. Fractions were analyzed by SDS-PAGE under non-reducing conditions, with the fraction indicated by blue asterisks used for cryo-EM studies. c, Western blot analysis of fraction 8, probed with anti-His, anti-gH, and anti-strep antibodies to detect UL141, gH, and UL116, respectively. d, Overview of the representative cryo-EM data processing workflow for the gH/UL116/UL141 complex.