Fig. 9. Cryo-EM structure validation and model quality assessment.

a, Map versus model FSC curves calculated with and without masking, using the Phenix package. Curves are shown for the HCMV gH/UL116/UL141 dimer (left) and the symmetry-expanded focused local refinement of the gH-UL116 interface (right). b, Stereo views of cryo-EM density maps for fragments of gH (left), UL116 (middle), and UL141 (right) from the 3-mer dimer, demonstrating the quality of the density. The cryo-EM density is displayed as a mesh. c, Stereo views of cryo-EM density maps for a fragment of gH and UL116 from the symmetry-expanded focused refinement of the gH-UL116 interface, illustrating the quality of the cryo-EM density. The density is shown as a mesh.