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[Preprint]. 2024 Oct 12:2024.10.11.617879. [Version 1] doi: 10.1101/2024.10.11.617879

Extended Data Fig. 7 |. SigL tetramers specifically label Siglec (WT)+ cells as an antibody surrogate.

Extended Data Fig. 7 |

a,b, Staining of Siglec-WT (wide type), Siglec-KO (knockout) and Siglec-R mutant on U937 cells using anti-Siglec antibodies and SigL tetramers bearing AF647 dye. Data for staining Siglec-7+ U937 cells are shown in (a); Data for staining Siglec-9+ U937 cells are shown in (b). c, Cell surface staining of Siglec-3 WT U937 cells with anti-Siglec-3 (clone WM53) and (SigLbio)4-SA-AF647 tetramers. d, Cell surface staining of Siglec-10 WT CHO cells with anti-Siglec-10 (clone 5G6) and (SigLbio)4-SA-AF647 tetramers. e, Cell surface staining of Siglec-2 WT CHO cells with anti-Siglec-2 (clone S-HCL-1) and (SigLbio)4-SA-AF647 tetramers.