| Genetic reagent (Mus musculus) |
C57BL/6J |
Charles River |
Strain code 631 |
|
| Genetic reagent (Mus musculus) |
Rph3a-/- mice |
Schlüter et al., 1999
|
– |
See section ‘Animals’ |
| Antibody |
Anti-chromogranin B (rabbit polyclonal) |
SySy |
259103 |
1:500 |
| Antibody |
Anti-RPH3A (mouse monoclonal) |
Transduction Laboratories |
– |
1:1000 |
| Antibody |
Anti-MAP2 (chicken polyclonal) |
Abcam |
ab5392 |
1:500 |
| Antibody |
Anti-Syn1 (rabbit polyclonal) |
#P610; SySy |
106 103 |
1:1000; 1:500 |
| Antibody |
Anti-VGLUT (rabbit polyclonal) |
SySy |
135302 |
1:500 |
| Antibody |
Anti-Homer1 (guinea pig polyclonal) |
SySy |
160 004 |
1:500 |
| Antibody |
Anti-Tau (xx, polyclonal) |
SySy |
314 004 |
1:1000 |
| Antibody |
Anti-VAMP2 (mouse monoclonal) |
SySy |
104 211 |
1:1000 |
| Antibody |
Anti-mCherry (mouse monoclonal) |
Signalway Antibody |
#T515 |
1:1000 |
| Recombinant DNA reagent |
pSyn(pr)Rabphilin3a(mus)-IRES2NLSCherryLL3.7 |
This paper |
- |
Generation of this reagent is described in Materials and methods section ‘Lentiviral vectors and infections’ |
| Recombinant DNA reagent |
pSyn(pr)EGFP-Glyrnlinker-Rabphilin3A-lentiFGA2.0 |
This paper |
- |
Generation of this reagent is described in Materials and methods section ‘Lentiviral vectors and infections’ |
| Recombinant DNA reagent |
pSyn(pr)mCherry-Glyrnlinker-Rabphilin3A(E50A,I54A)lenti-FGA2.0 |
This paper |
- |
Generation of this reagent is described in Materials and methods section ‘Lentiviral vectors and infections’ |
| Recombinant DNA reagent |
pSyn(pr)mScarlet-Glyrnlinker-Rabphilin3A(K648,653,660A)lentiFGA2.0 |
This paper |
- |
Generation of this reagent is described in Materials and methods section ‘Lentiviral vectors and infections’ |
| Recombinant DNA reagent |
pSyn(pr)mCherry-Glyrnlinker-Rabphilin3A(1-375)lenti-FGA2.0 |
This paper |
- |
Generation of this reagent is described in Materials and methods section ‘Lentiviral vectors and infections’ |
| Recombinant DNA reagent |
pSyn(pr)mCherry-Glyrnlinker-Rabphilin3A(D568N,D574N)lentiFGA2.0 |
This paper |
- |
Generation of this reagent is described in Materials and methods section ‘Lentiviral vectors and infections’ |
| Recombinant DNA reagent |
pSyn(pr)mCherry-Glyrnlinker-Rabphilin3A(S271A)-lentiFGA2.0 |
This paper |
- |
Generation of this reagent is described in Materials and methods section ‘Lentiviral vectors and infections’ |
| Recombinant DNA reagent |
pSyn(pr)hNPYPHluorin-N1lenti |
van de Bospoort et al., 2012
|
- |
Generation of this reagent is described in Materials and methods section ‘Lentiviral vectors and infections’ |
| Recombinant DNA reagent |
pSyn(pr)hNPYCherryLenti |
de Wit et al., 2009; Farina et al., 2015
|
- |
Generation of this reagent is described in Materials and methods section ‘Lentiviral vectors and infections’ |
| Recombinant DNA reagent |
pSyn(pr)HA-Tetx(E234Q)IRES2CherryDEST-lenti-fga2.0 |
Emperador Melero et al., 2017
|
- |
Generation of this reagent is described in Materials and methods section ‘Lentiviral vectors and infections’ |
| Software, algorithm |
MATLAB |
MathWorks |
- |
|
| Software, algorithm |
Prism |
GraphPad |
- |
|
| Software, algorithm |
ImageJ/Fiji |
ImageJ |
- |
|
| Software, algorithm |
Huygens Professional software |
Scientific Volume Imaging (SVI) |
- |
|
| Software, algorithm |
SynD |
Schmitz et al., 2011
|
- |
|
| Software, algorithm |
DCV fusion analysis |
Moro et al., 2021
|
- |
The DCV fusion MATLAB script is available in GitHub at https://git.vu.nl/public-neurosciences-fga/matlab-apps/fusionanalysis2 (Broeke, 2022) |
