Skip to main content
. Author manuscript; available in PMC: 2024 Oct 16.
Published in final edited form as: Methods Enzymol. 2024 Aug 27;705:311–345. doi: 10.1016/bs.mie.2024.08.001

Fig. 1. UDG deamination assay versus the RADD assay.

Fig. 1.

(A) Schematic representing the molecular process involved in traditional UDG-based deamination assays. (B) Schematic of the molecular process which results in real-time fluorescence reporting of APOBEC deamination in a single step through the RADD assay. FAM represents a 5′ 6-carboxyfluorescein fluorophore while IAB represents a 3′ Iowa Black® FQ quencher.

Figure adapted from Belica, C., Carpenter, M. A., Chen, Y., Moeller, N., Harris, R. S., & Aihara, H. (2024). A real-time biochemical assay for quantitative analyses of APOBEC-catalyzed DNA deamination. Journal of Biological Chemistry.