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Enzyme(+) mix.
| Enzyme (+) tube no. | 1 | 2 | 3 | 4 | 5 | 6 |
|---|---|---|---|---|---|---|
| 5 μM A3BctdDM | 4.5 μL | 4.5 μL | 4.5 μL | 4.5 μL | 4.5 μL | 4.5 μL |
| 20 μM EndoQ | 4.5 μL | 4.5 μL | 4.5 μL | 4.5 μL | 4.5 μL | 4.5 μL |
| BORF2 dilution | 4.5 μL (6.20 μM) | 4.5 μL (4.65 μM) | 4.5 μL (3.10 μM) | 4.5 μL (1.55 μM) | 4.5 μL (0.78 μM) | 4.5 μL (0 μM) |
| Enzyme storage buffer | – | – | – | – | – | – |
