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Michaelis-Menten enzyme kinetics reporter dilution series.
| Tube no. | 1 | 2 | 3 | 4 | 5 |
|---|---|---|---|---|---|
| DNase/RNase-free H2O | – | 12 μL | 12 μL | 12 μL | 12 μL |
| TC reporter | 24 μL (160 μM) | 12 μL (160 μM) | 12 μL (80 μM) | 12 μL (40 μM) | 12 μL (20 μM) |
| Final concentration | 160 μM | 80 μM | 40 μM | 20 μM | 10 μM |
