Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1987 Oct 15;247(2):293–297. doi: 10.1042/bj2470293

Light-induced changes in the content of inositol phosphates in squid (Loligo pealei) retina.

J E Brown 1, D C Watkins 1, C C Malbon 1
PMCID: PMC1148407  PMID: 2827625

Abstract

Illumination induced an increase in inositol polyphosphates, inositol 1,4-bisphosphate and inositol 1,4,5-trisphosphate, in the photoreceptors of the squid, Loligo pealei. There was a concomitant decrease in phosphatidylinositol 4,5-bisphosphate, but no light-induced change in any other phosphoinositide. None of these stimulus-induced changes were altered by treatment in vivo with pertussis toxin, which ADP-ribosylated a Mr-39000-peptide. These findings support the hypothesis that inositol 1,4,5-trisphosphate participates as either a messenger or a modulator in transduction in invertebrate photoreceptors.

Full text

PDF

Images in this article

294Fig. 1.
on p.294

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bentrop J., Paulsen R. Light-modulated ADP-ribosylation, protein phosphorylation and protein binding in isolated fly photoreceptor membranes. Eur J Biochem. 1986 Nov 17;161(1):61–67. doi: 10.1111/j.1432-1033.1986.tb10124.x. [DOI] [PubMed] [Google Scholar]
  2. Bolsover S. R., Brown J. E. Injection of guanosine and adenosine nucleotides into Limulus ventral photoreceptor cells. J Physiol. 1982 Nov;332:325–342. doi: 10.1113/jphysiol.1982.sp014416. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brown J. E., Rubin L. J. A direct demonstration that inositol-trisphosphate induces an increase in intracellular calcium in Limulus photoreceptors. Biochem Biophys Res Commun. 1984 Dec 28;125(3):1137–1142. doi: 10.1016/0006-291x(84)91402-5. [DOI] [PubMed] [Google Scholar]
  4. Brown J. E., Rubin L. J., Ghalayini A. J., Tarver A. P., Irvine R. F., Berridge M. J., Anderson R. E. myo-Inositol polyphosphate may be a messenger for visual excitation in Limulus photoreceptors. Nature. 1984 Sep 13;311(5982):160–163. doi: 10.1038/311160a0. [DOI] [PubMed] [Google Scholar]
  5. Calhoon R., Tsuda M., Ebrey T. G. A light-activated GTPase from octopus photoreceptors. Biochem Biophys Res Commun. 1980 Jun 30;94(4):1452–1457. doi: 10.1016/0006-291x(80)90582-3. [DOI] [PubMed] [Google Scholar]
  6. Cohen A. I. An ultrastructural analysis of the photoreceptors of the squid and their synaptic connections. I. Photoreceptive and non-synaptic regions of the retina. J Comp Neurol. 1973 Feb 1;147(3):351–378. doi: 10.1002/cne.901470304. [DOI] [PubMed] [Google Scholar]
  7. Downes C. P., Mussat M. C., Michell R. H. The inositol trisphosphate phosphomonoesterase of the human erythrocyte membrane. Biochem J. 1982 Apr 1;203(1):169–177. doi: 10.1042/bj2030169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fein A. Blockade of visual excitation and adaptation in Limulus photoreceptor by GDP-beta-S. Science. 1986 Jun 20;232(4757):1543–1545. doi: 10.1126/science.3487116. [DOI] [PubMed] [Google Scholar]
  9. Fein A., Payne R., Corson D. W., Berridge M. J., Irvine R. F. Photoreceptor excitation and adaptation by inositol 1,4,5-trisphosphate. Nature. 1984 Sep 13;311(5982):157–160. doi: 10.1038/311157a0. [DOI] [PubMed] [Google Scholar]
  10. Gilman A. G. G proteins and dual control of adenylate cyclase. Cell. 1984 Mar;36(3):577–579. doi: 10.1016/0092-8674(84)90336-2. [DOI] [PubMed] [Google Scholar]
  11. Gonzalez-Sastre F., Folch-Pi J. Thin-layer chromatography of the phosphoinositides. J Lipid Res. 1968 Jul;9(4):532–533. [PubMed] [Google Scholar]
  12. Irvine R. F., Anggård E. E., Letcher A. J., Downes C. P. Metabolism of inositol 1,4,5-trisphosphate and inositol 1,3,4-trisphosphate in rat parotid glands. Biochem J. 1985 Jul 15;229(2):505–511. doi: 10.1042/bj2290505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Katada T., Ui M. ADP ribosylation of the specific membrane protein of C6 cells by islet-activating protein associated with modification of adenylate cyclase activity. J Biol Chem. 1982 Jun 25;257(12):7210–7216. [PubMed] [Google Scholar]
  14. Litosch I., Fain J. N. Regulation of phosphoinositide breakdown by guanine nucleotides. Life Sci. 1986 Jul 21;39(3):187–194. doi: 10.1016/0024-3205(86)90529-1. [DOI] [PubMed] [Google Scholar]
  15. Majerus P. W., Connolly T. M., Deckmyn H., Ross T. S., Bross T. E., Ishii H., Bansal V. S., Wilson D. B. The metabolism of phosphoinositide-derived messenger molecules. Science. 1986 Dec 19;234(4783):1519–1526. doi: 10.1126/science.3024320. [DOI] [PubMed] [Google Scholar]
  16. Malbon C. C., Kaupp U. B., Brown J. E. Limulus ventral photoreceptors contain a homologue of the alpha-subunit of mammalian Ns. FEBS Lett. 1984 Jun 25;172(1):91–94. doi: 10.1016/0014-5793(84)80880-7. [DOI] [PubMed] [Google Scholar]
  17. Malbon C. C., Rapiejko P. J., Mangano T. J. Fat cell adenylate cyclase system. Enhanced inhibition by adenosine and GTP in the hypothyroid rat. J Biol Chem. 1985 Feb 25;260(4):2558–2564. [PubMed] [Google Scholar]
  18. Payne R., Corson D. W., Fein A., Berridge M. J. Excitation and adaptation of Limulus ventral photoreceptors by inositol 1,4,5 triphosphate result from a rise in intracellular calcium. J Gen Physiol. 1986 Jul;88(1):127–142. doi: 10.1085/jgp.88.1.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rapiejko P. J., Northup J. K., Evans T., Brown J. E., Malbon C. C. G-proteins of fat-cells. Role in hormonal regulation of intracellular inositol 1,4,5-trisphosphate. Biochem J. 1986 Nov 15;240(1):35–40. doi: 10.1042/bj2400035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Saibil H. R., Michel-Villaz M. Squid rhodopsin and GTP-binding protein crossreact with vertebrate photoreceptor enzymes. Proc Natl Acad Sci U S A. 1984 Aug;81(16):5111–5115. doi: 10.1073/pnas.81.16.5111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Stryer L., Bourne H. R. G proteins: a family of signal transducers. Annu Rev Cell Biol. 1986;2:391–419. doi: 10.1146/annurev.cb.02.110186.002135. [DOI] [PubMed] [Google Scholar]
  22. Szuts E. Z., Wood S. F., Reid M. S., Fein A. Light stimulates the rapid formation of inositol trisphosphate in squid retinas. Biochem J. 1986 Dec 15;240(3):929–932. doi: 10.1042/bj2400929. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Vandenberg C. A., Montal M. Light-regulated biochemical events in invertebrate photoreceptors. 2. Light-regulated phosphorylation of rhodopsin and phosphoinositides in squid photoreceptor membranes. Biochemistry. 1984 May 22;23(11):2347–2352. doi: 10.1021/bi00306a004. [DOI] [PubMed] [Google Scholar]
  24. Yoshioka T., Inoue H., Takagi M., Hayashi F., Amakawa T. The effect of isobutylmethylxanthine on the photoresponse and phosphorylation of phosphatidylinositol in octopus retina. Biochim Biophys Acta. 1983 Jan 4;755(1):50–55. doi: 10.1016/0304-4165(83)90271-4. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES