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. 2024 Oct 17;20:475. doi: 10.1186/s12917-024-04322-2

Table 1.

Nucleotide sequences used as primers in the PCR reaction to identify ESKAPE organisms

Organism Gene Primer sequences (5’-3’) Amplicon size a(bp) Reference
Enterococcus faecium sodA bF: GAAAAAACAATAGAAGAATTAT 215 [29]
cR: TGCTTTTTTGAATTCTTCTTTA
Staphylococcus aureus Stpahy-sau bF: AATCTTTGTCGGTACACGATATTCTTCACG 108 [30]
cR: CGTAATGAGATTTCAGTAGATAATACAACA
Klebsiella pneumoniae RcsA bF: GGATATCTGACCAGTCGG 176 [31]
cR: GGGTTTTGCGTAATGATCTG
Acinetobacter baumannii gryB bF: CACGCCGTA-AGAGTGCATTA 490 [32]
cR: AACGGAGCTTGTCAGGGTT
Pseudomonas aeruginosa 16 S rRNA bF: AATACCTTGCTGTTTTGACGTTAC 295 [33]
cR: TCAGTGTCAGTATCAGTCCAGGTG
Escherichia coli gadA bF: GATGAAATGGCGTTGGCGCAAG 373 [34]
cR: GGCGGAAGTCCCAGACGATATCC

aBase pairs, bForward primer, cReverse primer