TABLE 3.
Sequence of random isolates from the P3 populations in C7-10 cells
| No. and sequence of clones in librarya
| |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| −35a
|
−30
|
−25
|
−20
|
−15
|
−10
|
−5
|
+1
|
+5
|
|||||||||
| No.b | Sequence | No. | Sequence | No. | Sequence | No. | Sequence | No. | Sequence | No. | Sequence | No. | Sequence | No. | Sequence | No. | Sequence |
| 0 | UCUCC | 0 | CCUUU | 0 | AUUUC | 0 | GUAGA | 2 | GAUGC | 0 | CACCA | 1 | GGAUU | 4 | UAUCA | 1 | AGUCG |
| 8 | —AC—U | 3 | ——CGC | 4 | U—CCA | 5 | AC—AG | 5 | —G——— | 5 | ———A— | 4 | ————U | 11 | UCGAU | ||
| 1 | —U——U | 2 | G—C—C | 2 | GG—CU | 1 | AC——— | 1 | AG——— | 1 | AUCAC | 2 | —CA—— | ||||
| 1 | A——UU | 2 | ——G—— | 1 | ——CC— | 1 | A—CA— | 1 | AU——— | 1 | —UAU— | ||||||
| 2 | —AGG— | 1 | CGACG | 1 | UC-AU | 1 | —C——— | 1 | —AAU— | ||||||||
| 1 | A———A | 1 | —A——U | 1 | CCU—— | 1 | UCG—C | ||||||||||
| 1 | U—AGA | 1 | AGCC— | ||||||||||||||
a
Six or more clones were sequenced from each virus population after three passages in C7-10 mosquito cells. The wild-type sequence is at the top of each list and is underlined. For the other sequences, bases identical to the wild-type sequence are designated by a dash.
b
Number of times that particular sequence was found. The wildtype promoter sequence (underlined) for each 5 nucleotide block is the first listed. Numbers in bold indicate the sequences chosen for analysis of promoter activity and virus fitness.