TABLE 1.
Summary of cytokine RPAs
| Cytokine | Amt of cytokine detected ina:
|
||||
|---|---|---|---|---|---|
| PBMC (PMA/ ionomycin) | Primary B cells
|
LCLC | |||
| Resting (0 h) | EBV (20 h) | EBV (30 h) | |||
| IL-1α | + | − | − | − | + |
| IL-1β | +++ | + | + | + | − |
| IL-1Ra | ++ | ± | + | + | − |
| IL-2 | +++ | − | − | − | − |
| IL-3 | + | − | − | − | − |
| IL-4 | − | − | − | − | − |
| IL-5 | − | − | − | − | − |
| IL-6 | + | − | − | − | − |
| IL-7 | − | − | − | − | − |
| IL-9 | − | − | − | − | − |
| IL-10 | − | − | − | − | − |
| IL-12p35 | − | − | − | − | − |
| IL-12p40 | − | − | − | − | − |
| IL-13 | − | − | − | − | − |
| IL-14 | − | − | − | − | − |
| IL-15 | − | − | − | − | − |
| IFN-γ | +++ | − | − | − | − |
| IFN-β | + | ± | − | − | − |
| TNF-α | ++ | − | + | + | + |
| TNF-β (LT) | + | − | + | + | ++ |
| LTβ | + | ++ | + | + | − |
| TGF-β1 | + | + | + | + | + |
| TGF-β2 | + | − | − | − | − |
| TGF-β3 | + | ± | − | − | + |
| M-CSF | − | − | − | − | − |
| G-CSF | − | − | + | + | − |
| GM-CSF | + | − | − | − | − |
| LIF | + | − | − | − | − |
| SCF | − | − | − | − | − |
| hOSM | + | − | − | − | − |
| L32 | +++ | +++ | +++ | +++ | +++ |
| GAPDH | +++ | +++ | +++ | +++ | +++ |
| EBNA-2 | − | − | + | + | + |
a
Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Paque density gradient and were stimulated with PMA (50ng/ml) and ionomycin (500 ng/ml) for 6 h. Purified B cells were isolated by CD19 positive selection and were infected with EBV for 20 or 30 h. Cytokine levels in uninfected cells, EBV-infected cells, and an established LCL line (LCL C) were analyzed using 10 μg of total cellular RNA. The relative amount of cytokine detected is indicated by an arbritary score ranging from +++ (abundant cytokines) to ± or − (weakly expressed or absent cytokines, respectively). EBNA-2 status, determined by Western blotting, is also shown.