Fig. 23. Supramolecular tandem enzyme assay for real-time measurement of the proteolysis of enkephalin-type peptides with thermolysin, using Q7 and acridine orange as the reporter pair. (top right) Fluorescence intensity as a function of time at different concentrations of substrate, used to measure kcat/KM. (bottom right) Reaction rate as a function of inhibitor concentration, used to measure the inhibitory constant.71 Reproduced with permission from ref. 71. Copyright 2011 American Chemical Society.
