FIG. 3.

CS-RhMLV-E vector allows EGFP expression in rhesus macaques. Circulating leukocytes from rhesus macaques transduced with CS-RhMLV-E or HR'CMVEGFP were evaluated for fluorescent intensity of EGFP expression by flow cytometry. Granulocyte, monocyte, lymphocyte, RBC, and platelet populations were identified and gated according to size (forward scatter) and granularity (side scatter) and analyzed for EGFP expression. Representative results from CS-RhMLV-E-transduced rhesus macaque 95E132 (top, 18 weeks posttransplant) and HR'CMVEGFP vector-transduced rhesus macaque RC505 (bottom, 82 weeks posttransplant) are shown. The x axis represents logarithmic fluorescent intensity of EGFP; the y axis represents the forward scatter. Fifty thousand events were acquired for flow cytometric analysis. Samples were analyzed with a FACSCalibur machine (Becton Dickinson) under identical settings.