FIG. 2.

Restriction enzyme analysis of “G” 4.5-kbp donor-target full-site integration products. The circular half-site and full-site products (lane 1) are indicated on the left (lettering not in boldface type). The unincorporated donor on the 1.5% agarose gel is the fastest migrating band in lane 1. The XhoI restriction fragments in lane 2 resulting from the full-site products are indicated in boldface lettering (10.2, 7.4, and 4.6 kbp) on the left under XhoI (Fig. 1D). XhoI digestion of the circular half-site products (lane 2) results in circles with two different sizes of tails (X on right). MluI digestion (lane 3) produces the anticipated half-site and full-site fragments (not marked) (Fig. 1D). In lane 4, the combined XhoI/MluI digestion of the full-site product produces the anticipated 1:2:1 ratio of fragments migrating at 4.6, 4.2, and 3.7 kbp (Fig. 1D), respectively (at right, in boldface type). The two slowest migrating fragments in lane 4 are digested circular half-site products. Lane 5 contains molecular weight markers varying in size by ∼1 kbp (at right, not in boldface type).