FIG. 7.
Lack of DNase I protection by IN with a nonfunctional LTR donor. IN was assembled with a 3.6-kbp fragment containing a mutation (5′-CCCCAOH) at the U5 LTR end. Lane 1 contains naked DNA treated with DNase I. Lanes 2 to 5 contain 3, 5, 10, and 20 nM IN, respectively. Labeling is the same as in Fig. 5.