FIG. 2.

LP-BM5-infected B6 nude mice, which are normally MAIDS resistant, become MAIDS susceptible after reconstitution with CD154⁺ CD4⁺ T cells but not CD154⁻ CD4⁺ T cells. B6 nude mice were intravenously reconstituted with 2 × 10⁷ purified B6 w.t. CD154⁺ or CD154⁻ CD4⁺ T cells and 2 days later infected with LP-BM5 virus. At 8 weeks after LP-BM5 infection, only the CD154⁺ CD4⁺ T-cell-reconstituted mice had developed all of three positive disease readouts indicating MAIDS susceptibility (splenomegaly, hyper-IgG2a, and diminished spleen cell response to LPS), which were all statistically different from those obtained for control, reconstituted, uninfected mice and CD154⁻ CD4⁺ T-cell-reconstituted, LP-BM5-infected nude mice. Each bar represents a mean value ± standard deviation. P values were derived as detailed in the legend to Fig. 1. There were three mice for the reconstituted, uninfected group and four mice for the reconstituted, LP-BM5-infected group. This experiment is representative of three other experiments.