FIG. 5.
B6 CD40 k.o. mice do not develop LP-BM5-induced MAIDS after reconstitution with a cell preparation enriched for CD40+ DC and macrophages. CD40 k.o. mice were intravenously reconstituted with 5 × 106 spleen cells obtained form B6 SCID mouse donors; they were infected with LP-BM5 2 days later and assessed for the indicated MAIDS disease parameters at 11 weeks postinfection. Each bar represents the mean ± standard deviation. Serum IgG2a levels for LP-BM5-infected nonreconstituted CD40 k.o. mice are higher than those for uninfected k.o. mice, apparently in accordance with the published observation that LP-BM5 causes hyper-IgG1 and -IgE, (54) due to a virus-dependent but non-MAIDS-related isotype class switching in CD40 k.o. mice. P values were derived by the Student t test (NS [nonsignificant], P > (0.05). E:T, effector:target.