Fig. 4.

CCN2 variant weakens its stimulation effect on osteogenesis of bone marrow mesenchymal stromal cells (BMSCs). a ALP staining results of osteoblast differentiation of BMSCs infected with indicated lentivirus overexpressing wild-type or mutant hCCN2 (p.Arg22Pro), and ZsGreen lentivirus was used as a control (n = 9). Scale bar = 10 μm. b ALP quantification results of BMSCs infected with indicated lentivirus at day 7. Data are represented as mean ± SD. Student’s t test was used (n = 9). *P < 0.05; ***P < 0.001. RT-PCR analysis of CCN2 (c) and Alp, Runx2, Bglap (d) expression at day 7 during BMSCs differentiation. Data are represented as mean ± SD. Student’s t test was used (n = 4). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.000 1; ns, not significant