Fig. 7. Endocytosis blocking studies using super-resolution confocal microscopy in hCB₂R-CHO cells. Cells were stained for 10 min with 12 (A) or 16 (C). In order to minimize endocytosis, hCB₂R-CHO cells were pretreated for 30 min with 400 μM sucrose and 5 μg mL⁻¹ filipin and then incubated for 10 min either with 12 (B) or 16 (D). Association curve of 12 on the plasma membrane and internal membranes of hCB₂R-CHO cells without sucrose and filipin (E) and in the presence of both (F). Images are representative of three independent experiments. Scale bars, 10 μm. (E) and (F) demonstrate internal and external fluorescence over time.