Fig. 3.

The expression of neuropeptides in the spinal cord after different frequency EA. qRT-PCR was employed to evaluate the mRNA expression of POMC, PENK, and PDTN in the spinal cord. (A) Western blot analysis was performed to assess the protein expression levels of POMC, enkephalin and dynorphin A in the L4-L6 spinal cord. (B-G) All results were expressed as mean ± SEM and were statistically using one-way ANOVA followed by Tukey’s post-hoc test (n = 6). **p < 0.01, ***p < 0.001 compared to the Sham group; ^p < 0.05, ^^p < 0.01, & p < 0.05 compared to the cuff group