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Conditions for the synthesis of a panel of esterified Opt peptides. a10 mM MES–HCl buffer, pH 6.0. For details on reagent concentrations, solid-phase peptide synthesis of Opt, synthesis of diazo compounds 1–3 based on established routes,26,46,55 and portionwise diazo compound addition, see Schemes S1–S8 and Figures S1–S6.
