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ALV-E particle-associated RNA sequences expressed from quail cells inoculated with culture supernatant from azacytidine-stimulated CEF. Particle-associated RNA from day 29 supernatant was analyzed for the presence of full-length ev pol sequences. The 1.8-kbp fragment shown was generated from a nested PCR. RT−, RT-minus assay negative control; CEF DNA, CEF DNA PCR positive control.
