Fig 6: Gβ4 deficiency boosts primary macrophage phagocytosis.

A-D, Isogenic ΔGβ₄ and WT macrophages were derived from hiPSCs and subjected to biophysical and functional assays. A, Schematic of embryoid body generation from hiPSCs and differentiation of primary macrophages from GMPs. B-C, Membrane tethers were generated from WT and ΔGβ₄ macrophages using an optical trap. B, Quantification of membrane tether force. C, Quantification of membrane tether length. Data in B-C represent mean ± s.d. n ≥ 46 for each sample, pooled from 5 biological replicates. D, ΔGβ₄ and WT macrophages were osmotically shocked and allowed to expand to their full volume to assess total plasma membrane. Data represent mean ± s.d. n = 12 for each sample, pooled from 3 biological replicates. E, WT and ΔGβ₄ macrophages were challenged with IgG-coated MPs for 2 h and phagocytic uptake quantified by florescence microscopy. Data represent mean ± s.d., pooled from 5 biological replicates. Unpaired t-test (B, C, E) or two-way ANOVA (D), **** p< 0.0001.