FIG. 4.
Effect of E64d concentration on p21cip1 abundance in HCMV-infected cells. (A) LU cells were density arrested as described in Materials and Methods, HCMV infected (5 PFU/cell) or mock infected, and at 48 h p.i. treated with the indicated concentrations of E64d. Whole-cell lysates were prepared 6 h later, and 40 μg of protein from each lysate was resolved by SDS-PAGE. The proteins were transferred to nitrocellulose and probed with antibodies to p21cip1. The results from a representative experiment are shown. Mock, mock-infected cells in the absence of drug; Control, untreated HCMV-infected cells; 0, DMSO control, consisting of HCMV-infected cells treated with the same concentration of DMSO (0.1%) contained in the highest concentration of E64d. (B) Densitometric analysis of the results shown in panel A.