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. 2001 Apr;75(8):3636–3646. doi: 10.1128/JVI.75.8.3636-3646.2001

FIG. 1.

FIG. 1

Schematic representation of plasmid inserts tested for antiapoptosis activity in transient-transfection assays. LAT, shown at the top, indicates the relative location of the LAT promoter, the primary 8.3-kb LAT RNA, and the stable 2-kb LAT intron (black box). The region downstream of the 2-kb LAT contains a break and is not drawn to scale. APALAT contains the Moloney murine leukemia virus (MoMLV) long terminal repeat (LTR) driving expression of LAT (nucleotides 301 to 2659) from HSV-1 strain KOS. The remaining LAT fragments are all derived from HSV-1 strain McKrae. The portion of the LAT promoter in pEV (−161 to +76) is sufficient for high transcription levels in transient-transfection assays. pEV(pro−) is identical to pEV but without the promoter. The LAT fragments in pLAT3.3, pLAT2.6, pLAT2.5, and pLAT1.8 correspond to the UL37-UL38 LAT inserts in the LAT3.3A, LAT2.6A, LAT2.5A, and LAT1.8A mutant viruses shown in Fig. 5, respectively.