FIG. 2.

Efficiency of cell survival after transfection with LAT constructs. Deletion plasmids of LAT are shown in Fig. 1. CV-1 and neuro-2A (2A) cells were transfected with LAT constructs (4 μg of DNA) and pCMV–β-Gal (1 μg of DNA). Cells were treated with sodium butyrate (Na-But, 5 mM) or etoposide (Etop, 15 μM) to induce apoptosis. Cell survival was measured by counting β-Gal⁺ cells at 48 h after transfection as described previously (9, 10, 42). The number of β-Gal⁺ cells that were present in cultures transfected with pCMV–β-Gal plus pcDNA3.1 without etoposide or sodium butyrate treatment was set at 100%. The values are the means of five different experiments.