Figure 1.

NeuroART GUI for analysis and stimulation. A, GUI for real-time analysis. Key features highlighted include the augmented image with overlaid neuronal activity, automatically identified and manually selected neurons to closely monitor ΔF/F₀ traces, and the photostimulation trigger for optogenetic stimulation. B, Separate tab for holographic stimulation. Both the phase mask (phase pattern projected on the SLM) and the simulated intensity patterns are displayed for the convenience of the user.