FIG. 2.

Replication of wild-type HSV-1(F) and the γ₁34.5 mutants R3616 and H9813 in SK-N-SH cells. Confluent monolayers of cells were infected with viruses at 0.01 PFU per cell and incubated at 37°C. At various times postinfection, the cells were harvested, freeze-thawed three times, and titrated on Vero cells. Duplicate samples were analyzed in parallel at each time point, and the data represent assays from three experiments.