Exit of nucleocapsids from the nucleus. Enveloped EHV-1 (A), HSV-1 (B), ILTV (C), and PrV (D) particles in the perinuclear cisterna are shown. Release of EHV-1 (E), HSV-1 (F), and PrV (H) from the nuclear cisterna results from direct fusion. Simultaneous observation of primary envelopment (arrowhead) and deenvelopment (arrow) of HSV-1 particles is demonstrated in panel G. Capsidless HSV-1 particles (arrowhead) in the perinuclear cisterna (I) and fused with the outer lamella of the nuclear membrane (J [arrowhead]) were also observed. The bars represent 150 nm in panels A to F and H and 200 nm in panels G and I. N designates the nucleus.