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. Author manuscript; available in PMC: 2024 Oct 17.
Published in final edited form as: Mol Cancer Res. 2021 Jun 25;19(9):1534–1545. doi: 10.1158/1541-7786.MCR-20-0991

Fig 1. Apelin-derived from adipocytes promotes migration in APJ-dependent manner.

Fig 1.

(A) Representative western blot for apelin performed on whole cell lysates obtained from pre-adipocytes and mature adipocytes. (B) Apelin secreted by pre-adipocytes and mature adipocytes into the medium quantified using ELISA. (C) Representative images of 8 hr-transwell migration assay performed in OVCAR-5-EV and APJ lines and (D) its quantification. (E) Representative images of 8 hr-transwell migration assay performed in OVCAR-8 cell and (F) its quantification. The cells were allowed to migrate to control media (10% FBS) or Adipocyte-conditioned media (Adipo CM), as indicated.

Results were obtained from 3 independent experiments (mean±SEM). Statistical analysis was performed using student’s t-test for B, and one-way ANOVA followed by Tukey post hoc test for D,F. *P< 0.05; **P< 0.01;***P<0.001; ****P<0.0001.