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. Author manuscript; available in PMC: 2024 Oct 17.
Published in final edited form as: Mol Cancer Res. 2021 Jun 25;19(9):1534–1545. doi: 10.1158/1541-7786.MCR-20-0991

Fig 6. APJ activation promotes fatty acid utilization via AMPK-CPT1a pathway.

Fig 6.

(A,B) Western blots performed for pAMPK and total AMPK levels on whole cell lysates (WCLs) obtained from (A) OVCAR-5-EV and APJ cells, and (B) OVCAR-8 cells in the absence and presence of F13A treatment. (C,D) qRT-PCR performed for CPT1a expression using cDNA obtained from (C) OVCAR-5-EV and APJ cells and (D) OVCAR-8 cells with F13A treatment. The cells were cultured either in serum free medium (SFM) or in adipocyte conditioned medium (Adipo CM) as indicated. F13A dose used in these experiments ranged from 10–50 ng/mL. (E) Graphical summary of the paper illustrating that adipocyte-derived apelin promotes metastasis by modulating fatty acid uptake and utilization in OvCa cells.

Results were obtained from 3 independent experiments (mean±SEM). Statistical analysis was performed using one-way ANOVA followed by Tukey post hoc test for C,D. *P< 0.05; **P< 0.01; ****P<0.0001.