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Troubleshooting Guide for RNA Extraction
| Problem | Possible Cause | Solution |
|---|---|---|
| Low RNA yield | Insufficient starting sample | Collect RNA from at least 1,000 L4s or adults per sample for optimal yields. Adults yield more RNA compared to L4s |
| Incomplete resolubilization of RNA pellet | After obtaining RNA pellet, perform 10 m incubation at a higher temperature (i.e. ~50ºC) and store at −80ºC overnight to promote resolubilization |
