FIG. 5.

Analysis of virions released from wild-type virus (HSRV-2) and dimerization mutants. BHK/Bel-1 cells were transfected with 10 μg of the respective DNA, and the virus was pelleted 48 h later as described in Materials and Methods. (a) RPA of nucleic acid isolated from virions. The full-length probe and the protected fragment of 350 and 248 nucleotides (nt), respectively, are shown. The relative band intensities of each band compared with that of the positive control HSRV-2, determined using LabImage version 2.51, are shown at the bottom of the gel. (b) Western blot with foamy virus-infected antiserum. The 70- and 74-kDa doublet of the Gag protein is indicated. The relative band intensities of the 70- and 74-kDa Gag doublet compared with that of the positive control HSRV-2, determined using LabImage version 2.51, are shown at the bottom of the gel.