Figure 2. Identification of periostin-producing cells in the skin lesions of FADS mice.

(A) β-galactosidase (β-gal) positive cells in PDGFRα⁺ and EpCAM⁺ facial dermal cells of 4-week-old FADS/Postn^lacZ/+ mice and control/Postn^lacZ/+ mice. PDGFRα⁺-, EpCAM⁺-, CD31⁺-, and CD45.2⁺-gated cells are depicted. (B) β-gal positive cells in both RFP⁺PDGFRα⁺ and RFP⁻PDGFRα⁺ cells derived from 4-week-old FADS/Postn^lacZ/+/Rosa26^RFP/+mice and control/Postn^lacZ/+/Rosa26^RFP/+mice. (C) β-gal expression by treatment with or without 50 ng/mL IL-13 for 24 hours in both RFP⁻ (Ikk2⁺) and RFP⁺ (Ikk2⁻) primary cultured dermal fibroblasts. The data shown are the mean ± SEM of three fibroblast samples prepared separately from three mice. Similar results were obtained from three separate experiments. Statistical analysis was performed using a two-sided, paired Student t-test; *P < 0.05, **P < 0.01. NS, not significant.