| Component | Volume for 15 μl reaction |
|---|---|
| Sybr green | 7.5 μl |
| Forward primer (from 10 μM stock) | 0.15 μl |
| Reverse primer (from 10 μM stock) | 0.15 μl |
| cDNA | Variable |
| Water | Variable |
| Total volume | 15 μl |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.