Table 1 |.
Antibodies
| Primary antibodies for immunofluorescent staining | |||||
|---|---|---|---|---|---|
| Name | Host species | Clone number | Manufacturer and cat. no. | RRID | Dilution factor |
| CDX2 | Rabbit | EPR2764Y | Abcam (cat. no. ab76541) | AB_1523334 | 1:400 |
| cCASP3 | Rabbit | 5A1E | Cell Signaling (cat. no. 9664S) | AB_2070042 | 1:400 |
| COL4 | Mouse | COL-94 | Abcam (cat. no. ab6311) | AB_305414 | 1:100 |
| EPCAM | Goat | Polyclonal | R&D Systems (cat. no. AF960) | AB_355745 | 1:400 |
| FOXA1 | Mouse | Q-6 | Santa Cruz Biotechnology (cat. no. sc-101058) | AB_1124659 | 1:50 |
| HT2-280 | Mouse | TB-27 | Terrace Biotech (cat. no. TB-27AHT2-280) | AB_2832931 | 1:100 |
| hNUCL | Mouse | NM95 | Abcam (cat. no. ab190710) | — | 1:100 |
| KI-67 | Rabbit | D3B5 | Cell Signaling (cat. no. 9129S) | AB_2687446 | 1:200 |
| KRT13 | Rabbit | EPR3671 | Abcam (cat. no. ab92551) | AB_2134681 | 1:400 |
| MUCIN1 | Rabbit | EPR1023 | Abcam (cat. no. ab109185) | AB_10862483 | 1:400 |
| P63Α | Rabbit | D2K8X | Cell Signaling (cat. no. 13109) | AB_2637091 | 1:200 |
| PROSPC | Rabbit | Polyclonal | Millipore Sigma (cat. no. AB3786) | AB_91588 | 1:200 |
| SOX9 | Goat | Polyclonal | R&D Systems (cat. no. AF3075-SP) | — | 1:200 |
| SPB | Rabbit | Polyclonal | Seven Hills Bioreagents (cat. no. WRAB-48604) | — | 1:500 |
| NKX2.1 | Rabbit | Polyclonal | Seven Hills Bioreagents (cat. no. WRAB-1231) | AB_2832953 | 1:200 |
| ZEB1 | Rabbit | EPR17375 | Abcam (cat. no. ab203829) | — | 1:50 |
| Name | Host species | Conjugate | Manufacturer and cat. no. | RRID | Dilution factor |
| Anti-Mouse IgG (H+L) | Donkey | AF- 488 | Thermo Fisher Scientific (cat. no. A21202) | AB_141607 | 1:200 |
| Anti-Goat IgG (H+L) | Donkey | AF- 488 | Thermo Fisher Scientific (cat. no. A32814) | AB_2762838 | 1:200 |
| Anti-Rabbit IgG (H+L) | Donkey | AF- 488j | Thermo Fisher Scientific (cat. no. A21206) | AB_2535792 | 1:200 |
| Anti-Rat IgG (H+L) | Donkey | AF- 488 | Thermo Fisher Scientific (cat. no. A21208) | AB_141709 | 1:200 |
| Anti-mouse IgM Heavy Chain | Goat | AF- 488 | Thermo Fisher Scientific (cat. no. A21042) | AB_141357 | 1:200 |
| Anti-Mouse IgG (H+L) | Donkey | AF- 555 | Thermo Fisher Scientific (cat. no. A31570) | AB_2536180 | 1:200 |
| Anti-Goat IgG (H+L) | Donkey | AF- 555 | Thermo Fisher Scientific (cat. no. A32816) | AB_2762839 | 1:200 |
| Anti-Rabbit IgG (H+L) | Donkey | AF- 555 | Thermo Fisher Scientific (cat. no. A31572) | AB_162543 | 1:200 |
| Anti-Mouse IgG (H+L) | Donkey | AF- 647 | Thermo Fisher Scientific (cat. no. A31571) | AB_162542 | 1:200 |
| Anti-Goat IgG (H+L) | Donkey | AF- 647 | Thermo Fisher Scientific (cat. no. A21447) | AB_141844 | 1:200 |